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Identification of isolates by different laboratories and methods

Journal: Journal of the American Association for Laboratory Animal Science : JAALAS

Article Title: The Likelihood of Misidentifying Rodent Pasteurellaceae by Using Results from a Single PCR Assay

doi: 10.30802/AALAS-JAALAS-18-000049

Figure Lengend Snippet: Identification of isolates by different laboratories and methods

Article Snippet: Microbiology PCR Lab 1 Lab 2 Lab 1 Lab 2 BLAST References R. pneumotropicus (ATCC 35149; n = 2) R. pneumotropicus or R. heylii R. pneumotropicus R. pneumotropicus R. pneumotropicus R. pneumotropicus M75083.1 99% (nt 49–762) R. heylii (ATCC 12555; n = 1) NS NS R. heylii R. heylii R. heylii KX858303.1 100% (nt 11–745) AF012090.1 99% (nt 4–738) M. muris (ATCC 49577; n = 1) NS NS — — M. muris AF024526.1 99% (nt 70–762) Isolates Type 1 ( n = 5) R. pneumotropicus or R. heylii R. pneumotropicus R. pneumotropicus R. pneumotropicus R. pneumotropicus M75083.1 99% (nt 27–763) Type 2 ( n = 1) NS NS R. heylii R. heylii R. heylii KX858334.1 100% (nt 3–737) AF012090.1 99% (nt 4–738) Type 3 ( n = 6) FELASA – Pasteurella sp. not pneumotropica R. heylii — P. pneumotropicus JQ346058.1 100% (nt 14–762) Pasteurellaceae HF912264.1 99% (nt 1–708) Type 4 ( n = 2) NS NS — — M. muris AF024526.1 99% (nt 76–745) Type 5 ( n = 3) FELASA – M. muris — — M. muris KP278081.1 100% (nt 3–746) AF024526.1 98% (nt 13–759) Open in a separate window BLAST, BLAST analysis for 16s rRNA gene PCR and sequencing (approximately 700-750 bp; GenBank accession number for highest homology found in the BLAST databases is listed with the percentage similarity and the DNA nucleotide range that was covered); –, negative results; FELASA –, negative for the FELASA-recommended list of bacteria; NS, not submitted; nt, nucleotides.

Techniques:

High-resolution melting curve analysis of bacterial isolates

Journal: Journal of the American Association for Laboratory Animal Science : JAALAS

Article Title: The Likelihood of Misidentifying Rodent Pasteurellaceae by Using Results from a Single PCR Assay

doi: 10.30802/AALAS-JAALAS-18-000049

Figure Lengend Snippet: High-resolution melting curve analysis of bacterial isolates

Article Snippet: Microbiology PCR Lab 1 Lab 2 Lab 1 Lab 2 BLAST References R. pneumotropicus (ATCC 35149; n = 2) R. pneumotropicus or R. heylii R. pneumotropicus R. pneumotropicus R. pneumotropicus R. pneumotropicus M75083.1 99% (nt 49–762) R. heylii (ATCC 12555; n = 1) NS NS R. heylii R. heylii R. heylii KX858303.1 100% (nt 11–745) AF012090.1 99% (nt 4–738) M. muris (ATCC 49577; n = 1) NS NS — — M. muris AF024526.1 99% (nt 70–762) Isolates Type 1 ( n = 5) R. pneumotropicus or R. heylii R. pneumotropicus R. pneumotropicus R. pneumotropicus R. pneumotropicus M75083.1 99% (nt 27–763) Type 2 ( n = 1) NS NS R. heylii R. heylii R. heylii KX858334.1 100% (nt 3–737) AF012090.1 99% (nt 4–738) Type 3 ( n = 6) FELASA – Pasteurella sp. not pneumotropica R. heylii — P. pneumotropicus JQ346058.1 100% (nt 14–762) Pasteurellaceae HF912264.1 99% (nt 1–708) Type 4 ( n = 2) NS NS — — M. muris AF024526.1 99% (nt 76–745) Type 5 ( n = 3) FELASA – M. muris — — M. muris KP278081.1 100% (nt 3–746) AF024526.1 98% (nt 13–759) Open in a separate window BLAST, BLAST analysis for 16s rRNA gene PCR and sequencing (approximately 700-750 bp; GenBank accession number for highest homology found in the BLAST databases is listed with the percentage similarity and the DNA nucleotide range that was covered); –, negative results; FELASA –, negative for the FELASA-recommended list of bacteria; NS, not submitted; nt, nucleotides.

Techniques: